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Year : 2015  |  Volume : 2  |  Issue : 1  |  Page : 35-37

Establishment and study of passive heymann nephritis model

Division of Nephrology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China

Correspondence Address:
Yueyi Deng
No. 725 South Wanping Road, Shanghai 200032
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2225-1243.150010

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Objective: To discuss the establishment and stability of passive Heymann nephritis (PHN) in order to provide help for experimental study of membranous nephropathy (MN). Materials and Methods: We used 20 male rats of Sprague-Dawley weighing 120 to 150 g to prepare renal antigen Fx1A based on hypotonic lysis, Ca 2+ aggregation of contaminants and differential centrifugation and used six male New Zealand white rabbits for preparing anti-serum which was injected to SD rats to establish the model in different dosages (1 mL/100 g and 0.5 mL/100 g). The proteinuria of six model making rats were tested after 1st Week and then sacrificed and studied by direct immunofluorescence (IF) for deposition of rat IgG and electron microscopy (EM) in different time (1 st week and 7 th week ) with or without a booster injection. Results: After 7 days (1 st week) of model making, all six rats showed proteinuria (higher than 100 mg/dL). IF and EM study showed a typical pathological features of PHN (MN) but the intensity of IF intensity and electron dense deposits decreased in the rats without a booster injection. Conclusions: A PHN model could be successfully prepared according to the method we reported while a spontaneous remission may appear several weeks after the model making.

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